Journal: Nature Communications

Article Title: CD163 interacts with TWEAK to regulate tissue regeneration after ischaemic injury

doi: 10.1038/ncomms8792

Figure Lengend Snippet: ( a ) Immunoblotting of IL 14 days after femoral ligation ( n =5 per group) with graphs showing quantitation of densitometry for phosphorylated (p-p65)/total p65 (T-p65) and p52. ( b ) Immunoblotting of IL 28 days after femoral ligation ( n =5 per group) with graphs showing quantitation of densitometry for phosphorylated (p-p65)/total p65 (T-p65) and p52. ( c ) Immunostaining for Mac-3 (red) of IL of WT and CD163 −/− mice 3 and 5 days after femoral ligation. Scale bars, 100 μm. The graph shows the timecourse of Mac-3 staining ( n =5 per group). (d) Laser Doppler analysis of CD163 −/− mice 3, 7, 14, 21 and 28 days after femoral ligation with administration of scrambled control peptide (control) or NF-κB inhibitor NBD ( n =5 per group). ( e ) Immunoblotting of CD163 −/− IL at 14 days after femoral ligation with administration of scrambled control peptide (control) or NF-κB inhibitor (NBD) ( n =5 per group) with bar graphs showing quantitation of densitometry for phosphorylated/total p65, p52 and NICD on right. All bars show mean±s.e.m. * P <0.05 versus WT in a – c ; versus CD163 −/− control in d , e . Comparisons between groups were achieved using a two-sided student's t -test.

Article Snippet: For experiments involving inhibition of NF-kB signalling, CD163 −/− mice were randomized into two groups: mice subjected to intraperitoneal administration with IKK-NBD control peptide (100 μg day −1 , n =5, Enzo Life Sciences); or NF-kB inhibitor NBD (100 μg kg −1 day −1 , n =5, Enzo Life Sciences) daily for 14 days after HLI.

Techniques: Western Blot, Ligation, Quantitation Assay, Immunostaining, Staining

Journal: Toxicology

Article Title: Tumour necrosis factor-α (TNF-α) enhances dietary carcinogen-induced DNA damage in colorectal cancer epithelial cells through activation of JNK signaling pathway

doi: 10.1016/j.tox.2021.152806

Figure Lengend Snippet: JNK and NF-kB involvement in TNF-α induced DNA damage. ( A ) genotoxicity of HCT116, cells were treated with 0.13μM of Bortezomib (BZ) with TNF-α dose range of (0.01-100 pg/mL) for 24 h. (B) genotoxicity of HCT116, cells were treated with 30μM of JNK inhibitor (SP600125) with TNF-α dose range of (0.01-100 pg/mL) for 24 h. Statistically Significant differences are shown for comparisons between inhibitor treated cells Vs. untreated cells. Significance was assessed using one-way ANOVA (GraphPad Prism 8, (**** p < 0.0001) and (++++ p < 0.0001) compared to control. Data are presented as a mean and error bars represent the SEM for independent cultures(n=3).

Article Snippet: An NF-kB inhibitor, Bortezomib (BZ) (LC laboratories), was used at 0.13 μM 4.5 h prior to TNF-α treatment.

Techniques: Control